We don't ask you to trust us. We ask you to read the research.
Every ingredient in PregenBlend™ has been studied in peer-reviewed human clinical trials. Below, you'll find the science — complete with citations — so you can verify everything yourself.
Pregnenolone - 50mg Neurosteroid effects of Pregnenolone on memory and cognitive function Flood et al., PNAS 1992
The direct product of cholesterol conversion inside the mitochondria. The precursor to every steroid hormone in the body — testosterone, DHEA, cortisol, progesterone, estrogen.
As a neurosteroid, pregnenolone also acts directly on sigma-1 receptors in the brain, supporting memory, mood, and neuroprotection independent of its role as a hormone precursor.
Research on pregnenolone specifically increasing testosterone in healthy men is still emerging. Its primary proven role is as the upstream precursor that enables production — the factory input, not the output itself.
DHEA - 50mg Pubmed meta-analysis of 42 RCTs (2020) Pubmed meta-analysis of 42 RCTs (2020)
DHEA is the most abundant circulating steroid hormone in the human body. It converts directly to androstenedione and then to testosterone. DHEA levels peak at age 25 and decline by approximately 20% per decade.
Meta-analysis of 42 randomized controlled trials (PubMed, 2020) — "Overall results from 42 publications comprising 55 arms demonstrated that testosterone level was significantly increased after DHEA administration (WMD: 28.02 ng/dL, p = 0.00)."
KSM-66 Ashwagandha - 300mg Testosterone +96 ng/dL; cortisol reduced 30% vs placebo Wankhede et al., J Int Soc Sports Nutr, 2015
Cortisol is testosterone's primary antagonist — when cortisol rises, testosterone falls. KSM-66 ashwagandha has the strongest clinical evidence of any adaptogen for cortisol reduction, which in turn supports testosterone production.
Wankhede et al. (2015) — Journal of the International Society of Sports Nutrition. 64 male subjects, 8-week RCT. KSM-66 group (600mg/day) showed testosterone increase of +96 ng/dL vs. +18 ng/dL in placebo (p = 0.004). Cortisol significantly reduced.
Chandrasekhar et al. (2012) — Indian Journal of Psychological Medicine. 600mg KSM-66 daily for 60 days reduced cortisol by approximately 30% vs. placebo.
Vitamin D3 - 5,000 IU Supports testosterone synthesis; enhances D3 calcium direction Ito et al., Biosci Biotechnol Biochem, 2011
Vitamin D3 is technically a hormone, not a vitamin. It supports testosterone synthesis directly and reduces the rate at which testosterone converts to estrogen (aromatization). Approximately 42% of Americans are vitamin D deficient.
Zinc Glycinate - 25mg Testosterone doubled in zinc-deficient men after 6 months Prasad et al., Nutrition, 1996
Magnesium supports testosterone production by competing with SHBG (sex hormone-binding globulin) for binding to testosterone. When magnesium is present, more testosterone remains free and bioavailable. Magnesium also supports the enzymatic steps in testosterone biosynthesis.
Magnesium Glycinate - 200mg Testosterone significantly higher in Mg group; both free and total Cinar et al., Biol Trace Elem Res, 2011
Magnesium supports testosterone production by competing with SHBG (sex hormone-binding globulin) for binding to testosterone. When magnesium is present, more testosterone remains free and bioavailable. Magnesium also supports the enzymatic steps in testosterone biosynthesis.
Cinar et al. (2011) — Biological Trace Element Research. Both free and total testosterone were significantly higher in supplemented athletes vs. controls.
Methylated B6 (P-5-P) - 25mg Supports hormone methylation; reduces homocysteine MTHFR research — Daly et al., 2004
Up to 40% of men carry a mutation in the MTHFR gene that prevents proper processing of synthetic folic acid and cyanocobalamin (the cheap forms of B vitamins in most supplements). This impairs hormone methylation, DNA repair, and neurotransmitter production. Methylated forms bypass this genetic block entirely.
Standard supplements use folic acid and cyanocobalamin. These require conversion by the MTHFR enzyme. Men with MTHFR mutations cannot make this conversion efficiently. Pregen uses L-5-MTHF (methylfolate), P-5-P (active B6), and methylcobalamin — the forms the body can use directly.
Methylfolate (L-5-MTHF) - 400mcg Bioactive form; bypasses MTHFR gene mutations affecting 40% of men Stanger et al., 2009
Methylcobalamin (B12) - 500mcg Bioavailable form; supports energy, mood, nerve function Multiple RCTs
Up to 40% of men carry a mutation in the MTHFR gene that prevents proper processing of synthetic folic acid and cyanocobalamin (the cheap forms of B vitamins in most supplements). This impairs hormone methylation, DNA repair, and neurotransmitter production. Methylated forms bypass this genetic block entirely.
Standard supplements use folic acid and cyanocobalamin. These require conversion by the MTHFR enzyme. Men with MTHFR mutations cannot make this conversion efficiently. Pregen uses L-5-MTHF (methylfolate), P-5-P (active B6), and methylcobalamin — the forms the body can use directly.
NMN (Nicotinamide Mononucleotide) - 250mg NAD+ significantly elevated; physical endurance improved; biological age stable vs placebo Yi et al., GeroScience, 2023
NAD+ is the cellular energy currency that powers every metabolic process in the body — including testosterone biosynthesis. NAD+ levels decline approximately 50% between ages 20 and 60. NMN is a direct precursor to NAD+ and is the most clinically-studied NAD+ precursor available.
Supplementing with 300mg NMN/day for 90 days in men aged 40–60 nearly doubled telomere length in blood cells (Niu et al., 2021).
Omega-3 (EPA/DHA) - 1000mg+ Cardiovascular protection; reduces inflammation; supports hormone production via healthy fat substrate NEJM ASCEND Trial, 2018
Testosterone and all steroid hormones are fat-soluble. They are manufactured from cholesterol — itself a lipid. Adequate dietary fat, particularly EPA and DHA from fish oil, is essential for cell membrane integrity and hormone synthesis substrate availability.
Higher omega-3 blood levels are associated with a 4.7-year increase in life expectancy (Harris et al., 2021, American Journal of Clinical Nutrition). Omega-3s also reduce systemic inflammation, which can directly suppress testosterone production.
FAQ's
Get quick answers to some of the most asked questions by customers
Is Pregen a testosterone replacement therapy (TRT)?
No. Pregen does not add testosterone to your body — it supplies the raw materials your body uses to produce its own naturally. This is a supplement, not hormone therapy.
How long before I notice results?
Most customers report initial improvements in energy and sleep within 2–3 weeks. Measurable changes in body composition and libido typically emerge at 6–8 weeks of consistent daily use.
Can I take Pregen with other medications?
Consult your doctor before combining with any prescription medication, particularly statins, blood thinners, or hormone-related medications.
Why is pregnenolone included if DHEA is already in the formula?
Pregnenolone sits above DHEA in the hormone cascade. By supplying both, we support the complete pathway — not just one step. It's also the ingredient the brand is named after.
What makes Pregen different from other testosterone supplements?
Most testosterone boosters focus on late-stage support (zinc, fenugreek, Tongkat Ali). Pregen starts upstream — at pregnenolone, the very beginning of the process. We also include NMN for cellular energy and a high-potency Omega-3 softgel, making it a genuinely comprehensive daily system.
Is Pregen safe for long-term use?
All ingredients have established safety profiles at the doses used. The methylated B vitamins, D3/K2, zinc, magnesium, and Omega-3 are nutrients widely recommended for long-term supplementation. Pregnenolone and DHEA are generally well-tolerated; we recommend consulting your doctor for long-term use, especially for men with hormone-sensitive conditions.
Is this subscription locked in?
No. You can cancel, pause, or modify your subscription at any time in your account. No hidden fees, no cancellation penalties.